Antioxidant, antibacterial, and keratinocyte cell growth stimulation of herbal extracts used in traditional medicine in IRAN

Backgrounds and aims: Recently, herbal extracts attracted attention in various medical fields, including treatment of problematic wounds and dermatology. Based on these lines, this study was planned to investigate the antibacterial, antioxidant and epidermal keratinocyte growth stimulation of herbal extracts in the HEK cell lines. Methods: MTT viability test and BrdU Cell Proliferation Assay were used to study the cell proliferation effect of herbal extracts in Human epidermal keratinocyte cell line (HEK-001). 1,1diphenyl-2-picryhydrazyl (DPPH) radical scavenger and Reactive Oxygen Spices (ROS) assays were used as the antioxidant activity of herbal extracts. Results: MTT and BrdU assays showed a dose-dependent increased in the cell proliferation of the epidermal keratinocyte cell line, HEK-001, following exposure to herbal extracts. The present study shows that herbal extracts decrease DPPH radical scavenging activity. Moreover, the results indicated that herbal extracts significantly decreased ROS production in HEK-001in a dose-dependent manner.The herbal extracts showed antibacterial activity against both GramAmerican Journal of Research Communication www.usa-journals.com Rashidi, et al., 2014: Vol 2(9) 212 [email protected] positive bacteria (Bacillus subtilis and Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa). Conclusion: These results suggest that herbal extract may produce positive effects on the epidermal keratinocyte growth promotion partly through the regulation of free radicalscavenging and ROS activity in the HEK-001 cells.